Objective: This work aims to investigate the dynamic gene expression of Wnt family members in the oncogenesis of breast cancer in Tianjin albino 2 strain (TA2) mice. Methods: Clinical specimens of spontaneous breast cancers were harvested from 42 female TA2 mice, and mammary glands were collected from tissues of normal and pregnant TA2 mice. A gene chip was used to identify the dif-ferentially expressed genes between the tissues of breast cancer and the normal mammary gland. They were subsequently validated by real-time polymerase chain reaction and the change in the Wnt expressions in the oncogenesis of breast cancer in TA2 mice. Results: The average age of the TA2 mice at carcinogenesis was 336.706 ± 85.05 d, and the average frequency of delivery was 3.767 ± 1.79. Compared with the mammary gland of the normal TA2 mice, Wnt1, Wnt5a, and Wnt10b were obviously up-regulated in the breast cancer tissue of the TA2 mice. However, the expression of Wnt5b was down-regulated in the normal mammary gland and the breast cancer tissues of the TA2 mice. Conclusion: Wnt1, Wnt5a, and Wnt10b may promote the progression of breast cancer, whereas Wnt5b may inhibit the development of TA2 breast cancer.% 目的：研究乳腺癌生成过程中,Wnt 家族成员表达的变化规律.方法：收集自发乳腺癌小鼠42只,记录其详细资料.采用基因芯片筛选正常TA2小鼠乳腺组织和自发乳腺癌TA2小鼠乳腺癌组织中的差异表达基因,随后用Real-time PCR 进行验证,并检测TA2小鼠乳腺癌形成过程中相关基因表达的变化.结果：TA2自发乳腺癌小鼠平均见瘤年龄为(336.706±85.05)d,平均分娩次数为(3.767±1.79)次.分娩次数最多者为7次,其平均见瘤年龄只有251.5 d. TA2小鼠基因芯片结果发现Wnt家族成员中,Wnt1、Wnt10b、Wnt5a、Wnt5b在正常乳腺组织和乳腺癌组织中表达具有差异.Real time PCR 的结果证实了以上结果,并发现 Wnt1、Wnt10b、Wnt5a 在癌前病变和乳腺癌组织中表达高于正常乳腺组织,而 Wnt5b 在乳腺癌组织中表达低于正常乳腺组织.结论：Wnt1、Wnt5a、Wmt10b在TA2小鼠乳腺癌生成过程中起促进作用,而Wnt5b的作用可能是抑制小鼠乳腺癌的生成.
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