目的：研究CYP4F2基因多态性与蒙古族冠心病的关系以及蒙古族冠心病患者的临床特点。方法：对入选对象进行问卷调查，采用高温连接酶检测反应技术对234例蒙古族冠心病患者（冠心病组）和221例非冠心病患者（正常对照组）进行基因扩增和基因分型，分析CYP4F2基因的2个单核苷酸多态性（SNP）位点 rs1558139和rs2108622的多态性和蒙古族冠心病的关系。结果：与正常对照组比较，冠心病组男性比例（41.18％比67.95％）、吸烟史（32.13％比41.88％）、人体质量指数[BMI ，（21.66±4.53） kg／m2比（25.34±5.37） kg／m2]和甘油三酯水平[（1.66±0.90） mmol／L比（1.92±1.38） mmol／L ]均显著升高，而高密度脂蛋白胆固醇水平[（1.18±0.28） mmol／L比（1.07±0.29） mmol／L]显著降低， P＜0.05或＜0.01；冠心病组 CYP4F2基因 rs1558139和rs2108622的基因型频率和等位基因频率与正常对照组比较差异无显著性（ P＞0.05）。结论：蒙古族冠心病患者的临床特点是男性比例高、有吸烟史、高人体质量指数和高甘油三酯水平， CYP4F2基因 rs1558139和rs2108622多态性与蒙古族冠心病无明显关系。%Objective:To study the relationship between CYP4F2 gene polymorphism and coronary heart disease (CHD) in Mongolia patients ,and investigate clinical characteristics of these patients . Methods:All subjects received questionnaire . Gene amplification and genotyping were performed in 234 Mongolian CHD patients (CHD group) and 221 non-CHD pa‐tients (normal control group) using high temperature ligase detection reaction technique .The relationship between Mongo‐lian CHD and CYP4F2 gene polymorphisms of two single nucleotide polymorphism (SNP) sites (rs1558139 ,rs2108622) was analyzed .Results:Compared with normal control group ,there were significant rise in percentages of male (41.18% vs . 67.95% ) ,smoking history (32.13% vs .41.88% ) ,body mass index [BMI ,(21.66 ± 4.53 ) kg/m2 vs .(25.34 ± 5.37 ) kg/m2 ] and triglyceride level [ (1.66 ± 0.90) mmol/L vs .(1.92 ± 1.38) mmol/L] ,and significant reduction in level of high density lipoprotein cholesterol [ (1.18 ± 0.28) mmol/L vs .(1.07 ± 0.29) mmol/L] in CHD group , P<0.05 or <0.01.There were no significant difference in genotype and allele frequencies of rs 1558139 and rs2108622 between two groups . Conclusion:Clinical characteristics of Mongolian CHD patients include high male percentage ,smoking history ,high body mass index and high triglyceride level .CYP4F2 gene polymorphisms of rs1558139 and rs2108622 are not related to coronary heart disease in Mongolian patients .
展开▼
