谷氧还蛋白1和硫氧还蛋白1基因在云南乌金猪不同组织中的表达特点及L-组氨酸对其在氧化应激细胞中表达的影响

摘要

本试验旨在研究抗氧化基因谷氧还蛋白1(GRX1)、硫氧还蛋白1(TRX1)在云南乌金猪脑垂体、下丘脑、甲状腺、胸腺、胰腺、生殖腺、肝脏、皮肤、十二指肠、空肠、回肠11种组织中的差异表达,探讨L-组氨酸对乌金猪氧化应激细胞中GRX1、TRX1基因表达的调节作用.采用实时荧光定量PCR( RT-qPCR)方法检测乌金猪组织中GRX1、TRX1基因表达；以过氧化氢(H2O2)为氧化应激源建立氧化应激细胞模型,探讨L-组氨酸对GRX1、TRX1基因表达的调节作用.结果表明:1)GRX1、TRX1基因在乌金猪被检测组织中均有表达,肝脏表达量最高,其次是皮肤、空肠,其他组织中的表达量相对较低；2)乌金猪组织中TRX1基因表达量明显高于GRX1基因表达量；3)细胞培养结果表明,受到H2O2刺激时,氧化应激细胞中GRX1、TRX1基因产生过表达,L-组氨酸对氧化应激细胞或非应激细胞中GRX1、TRX1基因表达具有调节作用,其适宜浓度约为280 μg/mL.乌金猪GRX1、TRX1基因表达具有明显组织特异性,H2O2可诱导氧化应激细胞中GRX1、TRX1基因产生过表达,添加适宜浓度的L-组氨酸可以调节氧化应激细胞或非应激细胞中GRX1、TRX1基因表达.结果提示,通过营养途径可诱导乌金猪体内GRX1、TRX1基因的表达,这是缓解机体氧化应激损伤和增强抗氧化能力的一种有效方式.%This experiment was conducted to investigate the gene expressions of glutaredoxin 1 ( GRX1) and thioredoxin 1 ( TRX1) in pituitary, hypothalamus, thyroid, thymus, pancreas, gonad, liver, skin, duode-num , jejunum and ileum tissues and explore the regulation of L-histidine on gene expressions in oxidative stress cells of Yunnan Wujin pigs. Different expressions of GRXl and TRXl genes were determined by the method of real-time quantitative PCR (RT-qPCR) , and the regulation of L-histidine on GRX1 and TRX1 gene expressions was explored in oxidative stress cells induced by H2 O2. The results showed as follows: 1) GRX1 and TRX1 gene expressions in tissues of Wujin pigs were detected, the highest gene expressions of GRX1 and TRX1 were in the liver, and then skin and jejunum, and relatively lower gene expressions were in the other tissues. 2) TRX1 gene expression in tissues was higher than GRX1 gene expression for Wujin pigs. 3) The results of cell culture showed that when being induced by H2O2, oxidative stress cells produced the over expression of GRX1 and TRX1 genes. L-histidine had the regulation on GRX1 and TRX1 gene expressions in oxidative stress or no stress cells, being the adequate concentration of 280 μg/mL. GRX1 and TRX1 gene expressions had the obvi-ous tissue characteristics for Wujin pigs. H2O2 could induce the over expression of GRX1 and TRX1 genes in oxidative stress cells. Adding adequate concentration of L-histidine could regulate GRX1 and TRX1 gene ex-pressions in oxidative stress or no stress cells of Wujin pigs. It is suggested that this may be an important way of reducing the oxidative stress damage and improving the oxidation resistance, being GRX1 and TRX1 gene expressions of animal body induced by nutritional pathway for Wujin pigs.