猪伪狂犬病毒安徽株gE基因的克隆和序列分析

摘要

Porcine pseudorabies virus (PRV) isolates were obtained recently from infected pigs in Anhui area. The gE gene of the PRV isolates was amplified using a pair of primers based on GenBank database. The resulting PCR products were cloned and sequenced. The amplified fragments were 632 bp. The sequence homology and phylogenetic analyses were performed using DNAStar software. Eight PRV isolates from Anhui shared 96.8%-99.7%nucleotide sequence identity and 97.9%-100.0%amino acids identity among each other while these PRV isolates shared 97.0%-99.7%nucleotide sequence identity and 95.2%-100.0%amino acids identity with other reference strains from GenBank. Phylogenetic analysis indicated that PRV isolates from Anhui had a closer relationship with other domestic isolates than foreign reference strains.%参考GenBank中猪伪狂犬病病毒（Porcine pseudorabies Virus，PRV）gE基因的序列设计1对引物，对猪伪狂犬病病毒安徽分离株的gE基因进行PCR扩增和序列分析，扩增的目的片段大小为632 bp。应用DNAStar软件对gE基因序列核苷酸和推导的氨基酸序列的同源性分析并绘制遗传进化树。结果表明，猪伪狂犬病病毒安徽分离株之间的核苷酸同源性分别为96.8%~99.7％，氨基酸序列同源性为97.9%~100.0％，与GenBank收录的国内外其他地区的标准参考毒株的核苷酸同源性为97.0%~99.7％，氨基酸序列同源性为97.4%~100.0％。进化树分析表明，安徽分离株与目前国内流行的毒株在同一进化分支内，与国外分离株有一定差异。