水通道蛋白3在小鼠鼻腔中的表达

摘要

Objective: To investigate the expression of AQP3 in the nasal mucosa of mice. Methods: We detected the expression features of AQP3 between wild-type and AQP3-null mice in nasal mucosa with immunoblot and immunofluorescence. Results : Immunoblot result following SDS-PAGE showed that the expression of AQP3 positive bands was detected in the olfactory tissue samples of wild-type compare with negative bands of AQP3-null mice indicated the test mice were reliable. Immunofluorescence image revealed that AQP3 primary expressed in the support cells and basal cells with very strong staining in the olfactory epithelium and basal lateral membrane of Bowman gland cells with mild staining in the olfactory lamina prop-ria. AQP3 on the basal cells expressed through the transition of olfactory mucosa and airway mucosa. Expression of AQP3 was also detected on the basal cells in the nonsensory epithelium of vomeronasal organ. Immunofluorescence image of immediate dissociated cells of olfactory mucosa indicated that AQP3 could not express on neuron. Conclusion: AQP3 is extensively distributed on the non-neuron cells in olfactory mucosa, airway mucosa and vomeronasal organ, which may play a role in regulating the balance of water and glycerol in olfactory mucosa and maintaining the exciting of olfactory neurons, and addressed that it could play a very important role in the regeneration of nasal mucosa.%目的:研究水通道蛋白3(AQP3)在小鼠鼻腔中的表达.方法:应用免疫荧光和免疫印迹法研究野生型和AQP3基因敲除小鼠鼻腔嗅黏膜、呼吸道黏膜和犁鼻器中AQP3的表达特征.结果:免疫荧光结果表明,AQP3在小鼠嗅黏膜上皮层的基底细胞和支持细胞膜上表达强烈,并在嗅黏膜固有层中Bowman腺的基底外侧膜上表达；AQP3在嗅黏膜和上呼吸道黏膜组织中基底细胞的表达相延续；AQP3表达于犁鼻器非感觉黏膜上皮中的基底细胞.嗅黏膜组织急性分离后免疫荧光结果证实,AQP3不表达于嗅神经元.免疫印迹结果显示,野生型小鼠嗅黏膜组织液中可检测到AQP3的表达,而基因敲除小鼠嗅黏膜组织液中未能检测到相应条带.结论:AQP3广泛分布于鼻腔嗅黏膜、呼吸道黏膜和犁鼻器中的非神经元细胞,可能参与嗅黏膜组织局部水、甘油平衡的调节,在嗅神经元兴奋性维持、鼻腔黏膜组织的再生过程中发挥重要作用.