决明子对便秘小鼠结肠肌电和水通道蛋白3表达的影响

摘要

目的：探讨决明子对慢传输型便秘小鼠结肠肌电活动和水通道蛋白3（AQP3）在结肠黏膜表达的影响。方法40只雄性小鼠随机分为四组，对照组、便秘组、决明子组和酚酞组（阳性对照组）。建立小鼠慢传输便秘模型，用决明子，酚酞灌胃治疗决明子组和酚酞组。检测4组小鼠首便时间，6 h排便数量，结肠肌电信号，结肠黏膜AQP3的表达。结果决明子组与便秘组相比，首便时间缩短，6 h粪便数量增加（P＜0.05），结肠肌电慢波频率振幅均有极显著差异（P＜0.01），频率变异系数有显著差异（P＜0.05），结肠黏膜AQP3平均光密度有极显著差异（P＜0.01）。结论决明子能够有效地改善小鼠结肠运动功能，减少结肠黏膜AQP3的表达，对慢传输型便秘治疗具有显著疗效。%Objective To study Semen Cassiae on the colonic myoelectrical activity and the effect of the expression of aquaporin -3 (AQP3)inthecolonicmucosaofmicewithslowtransitconstipation.Methods 40malemicewererandomlydividedinto:control,constipa-tion,Semen Cassiae and phenolphthalein groups ( positive control group ) .The compound diphenoxylate tablets for intragastric mice was used to establish mice model of slow transit constipation ,and then the Semen Cassiae ,and phenolphthalein were given by intragastric administration to treat the mice of Semen Cassiae group and phenolphthalein group ,respectively.The constipation group were orally administered with the same volume of distilled water .The bowel transit function was measured by eosin propulsion test and the first defecation time and the number of red stool in the initiative 6 hours were recorded .BL-420F system was used for collecting change of myoelectrical signals of the mice colon . Immunohistochemical was used to detect the expression of AQP 3 in colon mucosa of mice .Results The effect of Semen Cassiae on the first defecation time and the number defecation of red stool in the initiative 6 hours had obviously changed .The grain number of red stool in 6 hours of constipation group was less than that of control group ,associated with prolong of the first defecation time (P<0.01).Compared with constipation group ,Semen Cassiae group and phenolphthalein group shortened the first defecation time and increased the grain number of the defecation red stool in 6 hours of the mice (P<0.05).The constipation group of colonic myoelectrical activity of mice slow-waves frequency was (7.78±1.59) Hz,slow-waves amplitude was (0.06±0.01) mV,and the Semen Cassiae group of slow-waves frequency was (3.32± 0.55)Hz,slow-waves amplitude was (0.07 ±0.02) mV.Compared with the constipation group,there were all significant differences (P<0.01).Compared with the Semen Cassiae group 〔(12.49±0.99)%〕,c slow-waves frequency variation coefficient of constipation group was significantly increased 〔(21.09±2.40)%,P<0.01〕.There was no significant difference in the slow-waves amplitude variation coeffi-cient between constipation and Semen Cassiae groups (16.91%±8.45%,vs 15.75%±4.09%,P>0.05).There was significant difference in the expression of the mean optic density of AQP 3 in colonic mucosa between constipation and Semen Cassiae groups (0.23±0.02 and 0.15± 0.01,P<0.01).Conclusions Semen Cassiae could improve motor function in mice colon ,reduce expression of AQP3 in colon mucosa,and has significant effects on the treatment of slow transit constipation .