利用掺杂多壁碳纳米管( MWNTs)的 Nafion 膜在玻碳电极上固定联吡啶钌( Ru(bpy)2+3),制得 Ru(bpy)2+3/ Nafion/ MWCNT 修饰电极。为了提高修饰电极的选择性,通过溶胶-凝胶技术,对该电极进一步修饰了溶胶-凝胶分子印迹膜,制得电化学发光-分子印迹传感器。优化了扫速、pH 值、富集时间等检测条件,传感器显示出既具电化学发光技术的灵敏性和分子印迹技术的选择性。在优化条件下,即 pH 7.0的磷酸盐缓冲溶液中,以100 mV/ s 扫速,富集5 min,对海洛因进行检测,在1.0×10-10~1.0×10-14 mol/ L 范围内有良好线性关系,检出限可达到4.0×10-15 mol/ L(S/ N=3)。传感器直接应用于唾液和尿液中海洛因的测定,回收率达到97%~104%。%An electrochemiluminescence (ECL) sensor with molecularly imprinted polymer (MIP) film was prepared for the determination of heroin. The sensor was prepared by re-modifying the molecular imprinting membrane onto the Ru ( bpy ) 2+3 modified glassy carbon electrode. The electrochemical and electrochemiluminescence behavior of the sensor was investigated. The proposed sensor displayed high sensitivity and excellent selectivity for the target molecule heroin. Under the optimum conditions (0. 1 mol/ L PBS (pH 7. 0) at a scan rates of 100 mV/ s and incubation time of 5 min), a linear response was observed with the concentrations of heroin from 1. 0 × 10-14 mol/ L to 1. 0 ×10-10 mol/ L and the detection limit was 4. 0 × 10-15 mol/ L. The sensor was successfully applied to the determination of heroin in urineand saliva samples with the recoveries from 97% to 104% .
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