稳定同位素标记衍生化结合液相色谱-串联质谱在生物样本中小分子代谢物分析的研究进展

摘要

稳定同位素标记衍生化 (Isotope coded derivatization,ICD)是通过化学衍生化反应对目标物的特定官能团进行同位素标记的技术,结合液相色谱-串联质谱(LC-MS/MS),可对具有相同标记反应特性的生物分子进行系统分析.该技术可以有效解决复杂生物基质分析中灵敏度不足与定量分析中同位素内标化合物有限的问题,近年来在代谢组学研究领域得到了广泛的应用.本文介绍了ICD方法及试剂的设计,对近年来针对羧基、氨基、羰基、巯基和羟基的ICD试剂以及结合LC-MS/MS在生物样本中小分子代谢物分析的研究进展进行了评述.%Stable isotope coded derivatization (ICD) is an isotope labeling technique for specific functional groups of the target analytes through chemical derivatization.ICD combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) enables systematically analyzing the biomolecules with the same labeling reaction characteristic.ICD technique can effectively solve the limited sensitivity in complex bio-matrices analysis and unavailability of isotope internal standards in quantitative analysis.In recent years, ICD technique has been widely used in metabolomics research field.Based on this background, the ICD technique and the design of ICD reagents are briefly described in this review.The recent advances in ICD reagents for carboxyl, amino, carbonyl, thiol and hydroxyl groups and their applications in the analysis of small molecule metabolites in bio-matrices with LC-MS/MS are reviewed.