A colorimetric method was developed for detection of mercury based on the inhibition of oxidation of peroxidase substrates. The as-prepared gold nanoclusters ( Au NCs), which has been stabilized and reduced by Glutathione (GSH), can effectively catalyze the H2 O2-TMB to generate a blue color signal. It is interestingly that Hg2+ can inhibit the oxidation of peroxidase substrates, thus causing a color diminished. Taking advantage of the inhibitive effect of Hg2+, a novel Hg2+ sensor has been developed. In this system, sensing conditions, including pH of the buffer solution, substrate concentration and time, were optimized. Under the optimal conditions, the probe showed a linear range of 10 - 300 nmol/ L ( R2 = 0. 997) with a detection limit of 6. 26 nmol/ L. In addition, this sensor exhibited good selectivity and sensitivity for Hg2+against other common environmental mental ions, providing a new method for water analysis.%以谷胱甘肽(GSH)为还原剂和稳定剂制备金纳米簇(Au NCs).Au NCs具有类过氧化物酶活性,可催化过氧化氢(H2 O2)和3,3',5,5'-四甲基联苯胺(TMB)的反应,使溶液变为蓝色;当溶液引入Hg2+,Hg2+吸附在金团簇表面,抑制其催化活性,使得反应体系颜色变浅.基于Hg2+的抑制作用设计了Hg2+比色传感器,考察了缓冲溶液pH值、 底物浓度及时间对检测Hg2+的影响.在最佳条件下,方法的线性范围为10～300 nmol/L(R2=0.997),检出限为6.26 nmol/L.本方法选择性好,灵敏度高,为水质分析提供了一种新方法.
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