稳定同位素稀释-超高效液相色谱-串联质谱法快速测定r植物油中16种真菌毒素

摘要

A fast, simple and cost-effective UPLC-MS / MS method was established for determination of 16 kinds of mycotoxins in vegetable oils with stable isotope dilution technique. Samples were extracted by acetonitrile-water-acetic acid 84: 15: 1(V/ V) and then diluted using water without any further clean-up steps. The mycotoxins were fully separated on a pentafluorophenyl column. Matrix effects were efficiently compensated by the [ 13 C]-labelled internal standards. The mean recoveries at three different concentration levels ranged from 74. 2％ to 105. 6％ , with RSD varied from 0. 3％ to 13. 9％ . Finally, the method was applied to analyze several kinds of vegetable oil samples. The method was simple, rapid, high sensitive and suitable for the determination of mycotoxins in vegetable oils.%建立了稳定同位素稀释-超高效液相色谱串联质谱法简单、快速、高效地检测植物油中16种真菌毒素的方法.植物油经乙腈-水-乙酸(84:15:1,V/V)提取并离心后,上清液用水1:1(V/V)稀释,高速低温离心去除油脂,过膜后,加入稳定同位素内标补偿基质效应干扰,以五氟苯色谱柱为分离柱,用甲醇和含有0.1％(V/V)甲酸的1 mmol/L乙酸铵溶液进行梯度洗脱,超高效液相色谱-串联质谱(UPLC-MS/MS)检测,内标标准曲线法定量测定植物油中的16种真菌毒素.16种真菌毒素的线性相关系数均大于0.9994,检出限为0.1～66.7μg/kg,定量限为0.3～200.0μg/kg.4种不同植物油基质中,3个浓度水平的加标回收率为74.2％ ～105.6％,相对标准偏差为0.3％ ～13.9％.采用本方法检测了市售38个植物油样品中的真菌毒素.本方法简便、快速、可靠,可用于植物油中16种真菌毒素的快速准确检测.