实时定量聚合酶链反应检测人半翼基因在宫颈病变中的表达及意义

摘要

Objective To investigate the expression of hWAPL mRNA and its significance in cervical lesions including cervicitis, CIN ( cervical intraepithelial neoplasia ) and cervical carcinoma. Methods Levels of hWAPL mRNA in 100 samples, including chronic cervicitis, CIN Ⅰ , CIN Ⅱ , CIN Ⅲ and cervical carcinoma ( 20 cases of each group ) were detected u-sing quantitative real - time PCR. Expression levels of p - actin were used as control to obtain the relative expression level of the target mRNA. Results The expression of hWAPL mRNA gradually increased in the order of chronic cervicitis, CIN Ⅰ , CIN Ⅱ , CIN Ⅲ and cervical carcinoma [ (1.05 ±0.68), (3.05 ±1.57), (4.48±2.25), ( 8. 71 ±7. 70 ) and ( 10. 49 ± 9. 82 ) ]. The expression of hWAPL mRNA in cervical carcinoma was significantly different from those in benign epithelia ( P ＜ 0. 05 for chronic cervicitis, CIN Ⅰ and CIN Ⅲ ). No significant difference in hWAPL mRNA expression was noted between cervical carcinoma and CIN Ⅲ ( P ＞0. 05 ). Conclusion hWAPL is expressed in tissues of chronic cervicitis, CIN Ⅰ , CIN Ⅱ, CIN Ⅲ and cervical carcinoma. The expression of hWAPL mRNA gradually increases in the order of chronic cervicitis, CIN Ⅰ , CIN Ⅲ , CIN Ⅲ and cervical carcinoma. The expression of hWAPL mRNA in cervical carcinoma is significantly higher than in chronic cervicitis, CIN Ⅰ and CIN Ⅱ. hWAPL may play a significant role in cervical carcinogenesis.%目的 探讨人半翼(hWAPL)基因在宫颈病变,包括慢性宫颈炎、宫颈上皮内瘤样变(CIN)和宫颈癌组织中的表达及意义.方法 用实时荧光定量聚合酶链反应(PCR)检测100例宫颈病变组织,包括慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ和宫颈癌组织各20例中hWAPL基因mRNA表达量,并经β-actin基因校正得到hWAPL基因mRNA相对表达量.结果 慢性宫颈炎组、CINⅠ组、CINⅡ组、CINⅢ组和宫颈癌组hWAPL基因 mRNA相对表达量逐渐升高,分别为(1.05±0.68)、(3.05±1.57)、(4.48±2.25)、(8.71±7.70)和(10 49±9 82).宫颈癌组和CINⅢ组与慢性宫颈炎组、CINⅠ组、CINⅡ组3组间比较差异均有统计学意义(P＜0.05＝;宫颈癌组与CINⅢ组比较,差异无统计学意义(P＞0.05).结论 hWAPL基因在宫颈组织,包括慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ和宫颈癌组织中均有表达.随着宫颈病变程度的加重hWAPL基因的表达量逐渐增加,在CINⅢ组和宫颈癌组中表达量明显高于慢性宫颈炎组、CINⅠ组和CINⅡ组.hWAPL基因的高表达可能在宫颈癌的发生中起重要作用.