Fn14与JAK／STAT在非小细胞肺癌EGFR外显子19缺失突变组织中的表达

摘要

Objective To explore the expression of fibroblast growth factor-inducible 14 ( Fn14 ) and Janus kinase/signal transducerand activator of transcription (JAK/STAT) in non-small cell lung cancer (NSCLC) tissues with EGFR exon 19 deletion. Methods In this study, 125 NSCLC cases with EGFR 19-Del were selected by amplification refractory mutation system. And the 30 cases of paired adjacent normal tissues were collected. Expressions of Fn14, p-JAK1 and p-STAT1 were detected in these samples using immunohistochemistry and the correlation of Fn14 expression with JAK/STAT expression was analyzed by statistics. Results The ex-pression of Fn14 was mainly located on cell membrane and cytoplasm, while the expression of p-JAK1 and p-STAT1 were mainly loca-ted in cytoplasm and cell nucleus. Compared to normal lung tissues, the higher expression rates of Fn14, p-JAK1 and p-STAT1 protein were 100. 0% ( 125/125) , 83. 2% ( 104/125) and 100. 0% ( 125/125) in NSCLC tissues with EGFR 19-Del ( P<0. 05) . There were significant differences of Fn14, p-JAK1 and p-STAT1 expression in histological grades, pTNM stages and lymph node metastasis ( P<0. 05) , while there were no significant differences in sex, age, smoking history and pathological types ( P>0. 05) . The correlation of Fn14 with p-JAK1 and p-STAT1 were obvious ( P<0. 05, r>0. 3) in pathological classification, histological grads and pTNM stage of NSCLC. Conclusion Fn14, p-JAK1 and p-STAT1 proteins are highly expressed in NSCLC with EGFR 19-Del, suggesting that the a-bove three proteins would be likely to associate with the development of NSCLC. The good correlations between Fn14 expression and p-JAK1, p-STAT1 hints that Fn14 may effect on the expression of p-JAK1, p-STAT1. All these provide important basis for the mecha-nism study of Fn14 affecting on the development of NSCLC with EGFR 19-Del.%目的：探讨成纤维细胞生长因子诱导14（ Fn14）和Janus激酶／信号转导子和转录激活子（ JAK／STAT）信号分子在非小细胞肺癌（ NSCLC） EGFR外显子19缺失突变（ EGFR 19-Del）组织样本中的表达情况。方法经突变扩增阻滞系统法筛选出125例EGFR 19-Del的NSCLC组织并收集30例配对癌旁组织，应用免疫组织化学法检测该组织样本中Fn14、p-JAK1和p-STAT1的表达，统计分析3种蛋白表达与临床病理特征的关系及三者之间的相关性。结果 Fn14阳性表达主要定位于细胞膜和胞质， p-JAK1、p-STAT1阳性表达主要定位于细胞质和胞核。 EGFR 19-Del的NSCLC组织中Fn14、p-JAK1、p-STAT1的阳性表达率分别为100．0％（125／125）、83．2％（104／125）和100．0％（125／125），均高于癌旁组织，差异有统计学意义（P＜0．05）。三者在性别、年龄、吸烟史、病理类型方面的表达差异均无统计学差意义（P＞0．05），在组织学分级、pTNM分期、淋巴结转移方面的表达差异均有统计学意义（ P＜0．05）。 Fn14与p-JAK1、p-STAT1在病理类型、组织学分级及pTNM分期方面表达均有很好的相关性（ P＜0．05，r＞0．3）。结论 Fn14、p-JAK1、p-STAT1在EGFR 19-Del的NSCLC组织中呈高表达，表明Fn14、p-JAK1、p-STAT1很可能与EGFR 19-Del 的NSCLC发生、发展有关；且Fn14可能促进p-JAK1、p-STAT1的表达，为进一步研究Fn14在EGFR 19-Del的NSCLC中的作用机制提供重要依据。