通心降脂滴丸对异丙肾上腺素致大鼠急性心肌缺血的保护作用

摘要

目的：探讨通心降脂滴丸对异丙肾上腺素（ISO）致急性心肌缺血的保护作用，为通心降脂滴丸的临床试验及临床应用提供药效学依据。方法取健康Wistar大鼠80只，乌拉坦麻醉，背位固定，测定标准肢体二导联心电图，筛选出心电图（ECG）正常动物60只。将60只大鼠随机均分为空白对照组，缺血模型组，普萘洛尔组，通心降脂滴丸低、中、高剂量组。通过皮下注射异丙肾上腺素建立大鼠心肌缺血损伤模型，检测各组大鼠血清中肌酸激酶（CK）、乳酸脱氢酶（LDH）、丙二醛（MDA）的含量和心肌组织中超氧化物歧化酶（SOD）活力，以及对异丙肾上腺素致心肌缺血后心电图的影响，评价通心降脂滴丸对异丙肾上腺素引起的心肌缺血的改善作用。结果缺血模型组大鼠心电图S－T段明显抬高，与空白对照组比较有显著性差异（ P<0.05）；与缺血模型组比较，通心降脂滴丸低、高剂量组大鼠的心电图S－T段均有明显下降。与空白对照组比较，缺血模型组LDH、CK活力、MDA含量均显著增高（ P<0.05）；与缺血模型组比较，通心降脂滴丸中剂量组MDA含量显著降低（ P<0.05），SOD活力显著增高（ P<0.05）；通心降脂滴丸高剂量组LDH、CK活力及MDA含量显著降低（ P<0.05），SOD活力显著增高（ P<0.05）。结论通心降脂滴丸可使心肌缺血大鼠心电图S－T段降低，缓解心肌缺血程度，减少心肌缺血后血清中LDH、CK的释放，以及心肌组织中MDA的产生，提高SOD活力，降低心肌损伤程度，对缺血心肌有保护作用。%Objective To explore the protective effects of Tongxin Jiangzhi Drops on isoproterenol(ISO) induced acute myocardial ischemia,providing its pharmacodynamic evidence for clinical trials and clinical application. Methods Taking 80 healthy Wistar rats were chosen and given urethane anesthesia and supine position fixing,the determination of limb standard two-lead ECG were conducted to screen out 60 normal animals. Totally 60 healthy Wistar rats were randomly divided into control group,ischemia model group,propra-nolol group,Tongxin Jiangzhi Drops low dose group,middle dose group and high dose group. Acute myocardial ischemia model in rats was established by subcutaneous injection of isoproterenol. Content of creatine kinase(CK) lactate dehydrogenase(LDH) and content of malondialdehyde(MDA) in serum and activity of the super oxide dismutase(SOD) in myocardial tissue homogenate were detected. Results Compared with the control group,the ischemia group's LDH,CK vitality,MDA content were significantly increased( P < 0. 05);Compared with the ischemia group,MDA content in the Tongxin Jiangzhi drops middle dose group was significantly lower( P < 0. 05), SOD activity was significantly increased( P < 0. 05);LDH,CK activity and MDA of the Tongxin Jiangzhi drops high dose group were significantly decreased( P < 0. 05),SOD activity was significantly increased( P < 0. 05). Conclusion Tongxin Jiangzhi Drops can reduce the release of LDH and CK in serum,reduce the production of myocardial tissue MDA,increase SOD activity and decrease the degree of myocardial injury,have a protective effect on acute ischemic myocardium.