酸枣仁汤对高架十字迷宫所致焦虑大鼠的影响

摘要

目的:探讨酸枣仁汤抗焦虑的药理作用机制.方法:建立高架十字迷宫大鼠焦虑模型,观察酸枣仁汤对焦虑大鼠行为的影响,ELISA法检测大鼠脑内海马五羟色胺(5-HT)、五羟吲哚乙酸(5-HIAA)、伽马氨基丁酸(GABA)、谷氨酸(GLU)、去甲肾上腺素(NE)的含量.结果:各给药组开放臂进入次数与进入时间与模型组比较显著提高(P＜0.05);各给药组进臂总次数与模型组比较差异无统计学意义(P＞0.05).酸枣仁汤大、小剂量组5-HT、5-HIAA、GABA、GLU、NE的含量均有一定变化.结论:酸枣仁汤对高架十字迷宫所致的大鼠焦虑有明显的治疗作用,其抗焦虑的机制可能与调节脑内海马神经递质的平衡有关,且酸枣仁汤小剂量组较大剂量组有更好的治疗效果.%Objective:To study the pharmacological mechanism of SuanZaoRen decoction (Spine Date Seed decoction) against anxiety.Methods:Model of anxious rats in elevated plus maze were established.Effects of SuanZaoRen decoction on anxious rats' behaviors were observed.The contents of 5-HT,5-HIAA,GABA,GLU and NE in hippocampus were determined by ELISA method.Results:Compared with the model group,the open-arm entry frequency and entry time of each intervention groups increased significantly (P＜0.05);compared with the model group,total arm entry frequencies of each intervention groups had no significant differences (P＞ 0.05).Contents of 5-HT,5-HIAA,GABA,GLU and NE in low and high dose groups of SuanZaoRen decoction all had certain changes.Conclusion:SuanZaoRen decoction can significantly relieve the anxiety of the rats caused by elevated plus maze,and its mechanism might be related to regulating the balance of neurotransmitters in intracephalic hippocampus,while low dose group of SuanZaoRen decoction show better effects compared with high dose group of SuanZaoRen decoction.