丹酚酸B配伍丹皮酚对H2O2诱导的人脐静脉内皮细胞氧化损伤模型Bcl-2、Bax、caspase-3mRNA及蛋白表达的影响

摘要

Objective To investigate the effects of Salvianolic Acid B with Paeonol on the Bcl-2, bax, caspase-3 mR-NA and protein expression in human vascular endothelial cells oxidative damage induced by H2O2. Methods H2O2 caused oxidative damage model of human umbilical vein endothelial cells. SYBR Green RT-PCR was used to detect expression change of related genes Bcl-2, Bax and caspase-3 mRNA on endothelial cells apoptosis after administration; Western blot was used to detect expression of proteins Bcl-2, Bax, caspase-3 related to endothelial cell apoptosis. Results Bcl-2 mRNA level was increased by Salvianolic Acid B with Paeonol in the damaged cells, while the bax, caspase-3 gene expression was decreased, it's more consistent with the results of Western blot analysis of protein content, and data was statistically significant (P < 0.01). Conclusion Salvianolic Acid B with Paeonol protect the vascular endothelial cells through regulating the expression of Bcl-2, Bax mRNA and protein expression and inhibiting the caspase-3 activation of the downstream molecules.%目的 探讨丹酚酸B配伍丹皮酚对过氧化氢(H2O2)诱导的人脐静脉内皮细胞氧化损伤模型Bcl-2、Bax、caspase-3 mRNA及蛋白表达的影响.方法 采用H2O2造成人脐静脉内皮细胞氧化损伤模型,利用SYBR Green RT-PCR方法检测药物作用内皮细胞后凋亡相关基因Bcl-2、Bax、caspase-3 mRNA的表达变化,Western blot法检测内皮细胞凋亡相关蛋白Bcl-2、Bax、caspase-3的表达.结果 丹酚酸B配伍丹皮酚能够使损伤内皮细胞中Bcl-2基因mRNA水平上升,而Bax、caspase-3基因的表达降低,与Western blot方法检测蛋白含量的结果较为一致,且数据比较差异有高度统计学意义(P < 0.01).结论 丹酚酸B配伍丹皮酚是通过调节Bcl-2、Bax基因及蛋白的表达,抑制下游分子caspase-3的活化而起到保护血管内皮细胞的作用.