IL-10与IL-12在银屑病患者系统血液快速固有免疫反应中的表达

摘要

目的:检测银屑病患者IL-10及IL-12在体外系统血液快速固有免疫反应体系中的表达.方法:将灭活大肠杆菌(3×108/mL)0.2 mL做为免疫原加入抗凝全血细胞悬液0.2 mL和血浆0.3 mL中,37℃水浴1 h,离心,取上层血浆,固相夹心法酶联免疫吸附试验(ELISA)测定血浆IL-10及IL-12含量.结果:银屑病血液经免疫原激活后IL-12含量明显上升,IL-10含量无明显上升,但正常人免疫原激活后IL-12和IL-10含量都明显上升.银屑病血液经免疫原激活后IL-12/IL-10比值增高.结论:银屑病患者血浆中IL-12与IL-10细胞因子水平的异常是固有免疫反应亢进的一种表现,可能与银屑病的系统免疫学发病机制相关.%Objective: To detect the expression of IL- 10 and IL- 12 in patients with psoriasis by systematic hematogenic immunoreaction.Method: Escherichia coli suspension (3 × 108/mL) 0.2 mL was added to anticoagulate whole blood with citric acid and 0.3 mL plasma, followed by 1h incubation at 37℃.The expression of IL- 10 and IL- 12 in plasma was determined by ELISA.Results: The IL- 12 level in psoriasis patients was significantly incrossed after immunogen activation, while the IL- 10 level was not significantly increased.In healthy subjects, both levels of IL- 10 and IL- 12 were increased significantly after immunogen activation.The plasma IL- 12/IL- 10 ratio increased after immunogen activation in psoriasis patients.Conclusion: The abnormal levels of IL- 10 and IL12 show the over - expression of innate immune in psoriasis patients, which may play an important role in systematic immune pathogenesis of psoriasis.