苦参碱对人视网膜母细胞瘤细胞体外生长增殖的影响

摘要

OBJECTIVE To study the effect of matrine on the apoptosis of retinoblastoma cells.METHODS Retinoblastoma cells were cultured in 0.4 mmol/L matrine for 12 hours, 24 hours or 48 hours respectively, and the control group was cultured in equivalence of the standard the nutrient solution RPMI-1640 at concentrations of 10％ fatal bovine serum substitute for matrine.The apoptosis rate was evaluated by flow cytometry.RESULTS Apoptotic cells were observed after 12 h, and then developed in a time-dependent manner after 48 hours.The difference was statistically significant compared with the control group (P＜0.05).Followed by 12 h of matrine treatment, the ratio of viable apoptotic cells to total ones was 89.20％, and then decreased to 78.97％ and 76.12％ by 24 h and 48 h respectively.CONDLUSIONS Matrine could inhibit the proliferation of retinoblastoma cells though induction of its apoptosis in time-dependent manner.%目的 探讨苦参(matrine,Ma)对体外培养的人视网膜母细胞瘤(retinoblastoma,Rb)细胞株增生的抑制作用及对该细胞凋亡的影响.方法 将0.4mmol/L浓度的苦参碱作用于体外培养的人视网膜母细胞瘤细胞株12 h、24 h、48 h,对照组以等量含10%类标准胎牛血清的RPMI-1640培养液替代苦参碱.观察苦参碱对视网膜母细胞瘤细胞生长增殖的影响,流式细胞仪检测细胞凋亡率.结果 实验组12h后可观察到细胞开始凋亡,48h内呈时间依赖性.实验组与对照组比较差异具有统计学意义(P<0.05).苦参碱作用Rb细胞12 h、24 h、48 h后早期凋亡细胞分别占凋亡细胞的89.20%、78.97%和76.12%.结论 Ma对Rb细胞的增殖有抑制和凋亡诱导作用,其促进凋亡的能力存在时间依赖性.