以梭鱼金属硫蛋白基因表达监测海洋重金属污染

摘要

In this study, a 132-bp sequence corresponding to a 44-amino acid sequence of metallothionein gene was obtained, and then a quantitative real-time reverse transcription polymerase chain reaction assay was developed for quantification of MT mRNA normalized to β-actin in redeye mullets. The method was applied to detect MT mRNA expression in redeye mullets from Nandaihe and Dashentang in Bohai Bay, and the results showed that MT mRNA expression in redeye mullets was higher significantly from Nandaihe (0.012 ± 0.0064 copies/copy β-actin in males and 0.0099 ± 0.0042 copies/copy β-actin in females) than that from Dashentang (0.0017 ± 0.0011 copies/copy β-actin in males and 0.0014 ± 0.00095 copies/copy yff-actin in females) which was consistent with the heavy meials concentrations, indicating that MT mRNA can be a promising biomarker for monitoring metals pollutions in coastal environment.%分离了梭鱼金属硫蛋白132个碱基对应44个氨基酸的部分基因序列,以此为基础建立了分析梭鱼金属硫蛋白基因表达的实时定量PCR方法,并用于分析渤海南戴河和大神堂近岸海域野生梭鱼金属硫蛋白基因的表达.结果表明,南戴河野生梭鱼金属硫蛋白基因的表达水平(雄鱼:0.012±0.0064 copies/copy β-actin;雌鱼:0.0099±0.0042 copies/copy β-actin)明显高于大神堂野生梭鱼的表达水平(雄鱼:0.0017+0.0011 copies/copy β-actin;雌鱼:0.0014±0.00095 copies/copy β-actin).该结果与两地野生梭鱼体内重金属残留水平相一致,提示梭鱼金属硫蛋白基因可作为监测海洋重金属污染的敏感标志物之一.