Manufacturing Method for Metallothionein, a Biomarker for Heavy Metal Contamination

摘要

The invention thionine (metallothionein; MT) into a fish metal in vivo exposure to heavy metals MT is amplified so figure out whether the contamination of drinking water fish such as farmed fish of heavy metals exhibit characteristics useful biomarkers generated according to the expression of a gene ( It relates to an efficient method for producing thionine a metal which can be used as a biomarker). ; So this according to the invention after anesthesia of the fish after injection dissolved heavy metal salt high concentration solution such as cadmium chloride in saline made with high concentrations induction of thionine with metal easily by ensuring that fish is not our company through proper hygiene and well as induction extraction and blood bypasses the homogenisation extraction process that is accompanying the organs and institutions such as the liver (liver) and gills (gill) through a complex anatomical course also in to recover the thionine of the period derived metal in the fish body can be easily harvested by the thionine as a metal induced into the blood of the fish recovered enough to be used as a simple anion exchange chromatography process thionine antibody standard for the creation and analysis with specific species of fish from serum through a metal refinery. Therefore, efficient method of producing thionine in a metal present in the present invention is fish metal to be produced as a single integral source-based technology to develop biomarkers search technique on whether heavy metals in drinking water fish such as farmed fish, as the product according to this method through the use of methionine zloty it is expected to be accelerated method development of the immune and enzyme immunoassay sensors for heavy metal contamination, such as bio-indicators. ; Heavy metal pollution, biomarkers, as metal thionine, edible fish