Occurrence and distribution of six heavy metals and three antibiotics were investigated in a sewage treatment plant in Shanghai using inductively coupled plasma mass spectrometer (ICP-MS) and solid-phase extraction combined with high-performance liquid chromatograph tandem mass spectrometer (SPE-HPLC-MS/MS), respectively. Quantitative PCR (qPCR) was used to determine the distribution and removal of seven erythromycin resistance genes (ERY-ARGs) during the wastewater treatment process. The results showed that sulfamethoxazole (SMX), erythromycin (ERY) and tetracycline (TC) were detected, and their respective removal efficiency was low in the range from 3% (ERY) to 36% (TC). Heavy metals of Cr、Cu、Zn and Pb were detected with concentrations in the ranges of 136.9~235.5, 7.1~37.4, 18.1~98.4 and 143.1~383.0µg/L, respectively. Almost completely removal of Zn was found by the two-stage anoxic/oxic (A/O) process, while the elimination rates for Cu, Pb and Cr were 48%, 43% and 18%, respectively. Additionally, all ERY-ARGs were detected in the range between 9.28×103copies/L (ermA) and 1.83×108copies/L (ereA) in raw influent and were significantly reduced (1.19~3.97logs) in the wastewater treatment process. Based on the correlation analyses, the concentrations of ERY-ARGs exhibited significantly positive correlation (P<0.05) with ERY. Also, the concentration ofereA genes was strongly correlated with those of Cu, Zn and Pb (P<0.05), indicating that the presence of ERY played an important role in the evolution of ERY-ARGs, while heavy metals possibly exert selective pressures on the ERY-ARGs.%采用电感耦合等离子体质谱(ICP-MS)和固相萃取-高效液相色谱串联质谱(SPE-HPLC-MS/MS)分别检测分析了上海某污水处理厂中6种重金属和3种抗生素的含量水平和分布特征,采用实时荧光定量PCR检测分析了7种红霉素抗药性基因(ERY-ARGs)在污水处理厂中的分布变化.结果表明,抗生素磺胺甲恶唑(SMX)、红霉素(ERY)和四环素(TC)在污水处理厂中均被检出,两段A/O工艺对其去除效果较差,去除率为3%(ERY)~36%(TC).重金属Cr、Cu、Zn和Pb在污水中被检出,浓度范围分别为136.9~235.5、7.1~37.4、18.1~98.4和143.1~383.0µg/L,两段A/O工艺对Zn基本完全去除,但对Cu、Pb和Cr的去除率分别为48%、43%和18%.目标ERY-ARGs在污水处理厂中均被检出,其在原水中的浓度为9.28×103(ermA)~1.83×108(ereA)copies/L,两段A/O工艺对其具有较好的去除效果,降低幅度可达1.19~3.97个对数浓度.通过相关性分析可知, ERY-ARGs与ERY之间具有显著相关性(P<0.05),而酯酶基因ereA与Cu、Zn和Pb之间也呈现出较好的显著相关性(P<0.05),表明污水中ERY对ERY-ARGs的演变产生具有重要影响,而重金属对ERY-ARGs也可能存在潜在的选择性作用.
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