首页> 外国专利> PLANT GENE FAMILY PCR THAT CONFERS TOLERANCE TO HEAVY METAL AND ORGANISM TRANSFORMED WITH THE SAME GENE FAMILY WHICH REMOVES HEAVY METALS FROM THE POLLUTED SOIL OR WATER

PLANT GENE FAMILY PCR THAT CONFERS TOLERANCE TO HEAVY METAL AND ORGANISM TRANSFORMED WITH THE SAME GENE FAMILY WHICH REMOVES HEAVY METALS FROM THE POLLUTED SOIL OR WATER

机译:植物基因家族PCR,可耐受重金属和有机体的转化,该基因组可从污染的土壤或水中去除重金属

摘要

PURPOSE: A plant gene family Pcr that confers tolerance to heavy metal and an organism transformed with the same gene family are provided, which transformed organism removes heavy metals from the polluted soil or water, so that the transformed organism can environment-friendly purify the polluted environments. CONSTITUTION: The plant gene family Pcr that confers tolerance to heavy metal is provided, wherein the gene family Pcr is selected from AtPcr1, AtPcr2, AtPcr8, AtPcr9, AtPcr10 and OsPcr1; the gene family Pcr expresses a protein having the amino acid sequence which has 29% or more homology to the total amino acid sequence of a protein AtPcr1, and a protein containing a domain having the amino acid sequence which has 35% or more homology to the amino acid sequence of a transmembrane domain of the protein AtPcr1; the transmembrane domain of the protein AtPcr1 has tolerance to cadmium; and the protein AtPcr1 is tissue-specifically expressed. A recombinant vector pYES2/NTC-Pcr or PGA1535-Pcr comprises the gene family Pcr. The transformed organism with the recombinant vector pYES2/NTC-Pcr or PGA1535-Pcr is provided, wherein the transformed organism is an eukaryote such as animal, plant and yeast and prokaryote.
机译:目的:提供对重金属具有耐受性的植物基因家族Pcr,并提供一种用相同基因家族转化的生物,该转化生物可以从污染的土壤或水中去除重金属,从而使转化的生物能够环保地净化污染的生物。环境。组成:提供赋予重金属耐受性的植物基因家族Pcr,其中该基因家族Pcr选自AtPcr1,AtPcr2,AtPcr8,AtPcr9,AtPcr10和OsPcr1。基因家族Pcr表达具有与蛋白质AtPcr1的总氨基酸序列具有29%或更高同源性的氨基酸序列的蛋白质,以及含有具有与氨基酸序列具有35%或更高同源性的氨基酸结构域的蛋白质。蛋白AtPcr1的跨膜结构域的氨基酸序列; AtPcr1蛋白的跨膜结构域对镉具有耐受性。 AtPcr1蛋白在组织中特异性表达。重组载体pYES2 / NTC-Pcr或PGA1535-Pcr包含基因家族Pcr。提供了具有重组载体pYES2 / NTC-Pcr或PGA1535-Pcr的转化生物,其中转化生物是真核生物,例如动物,植物,酵母和原核生物。

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