高效液相色谱蒸发光散射检测法测定乳制品中乳果糖

摘要

A method for determination of lactulose in dairy products by high-performance liquid chromatography (HPLC) was established.Protein in dairy products were mostly eliminated by adjusted pH to 1.7 and 4.5,separated on NH2 column with a mixed solution of acetonitrile-water as mobile phase by gradient elution,and detected with a evaporative light-scattering detector (ELSD).The sugar had good linearities with 0.01 ～ 10.0 mg/mL.The recovery were 92.0％ ～ 103.5％,the relative standard deviations(RSD) were 2.98％ ～ 5.56％,the minimum detectable values were 0.04 mg/g.The method can be used for the routine analysis oflactulose in dairy products.%建立了高效液相色谱-蒸发光散射检测法测定乳制品中乳果糖的方法.通过调节样品溶液的pH值到1.7和4.5除去样品中蛋白质,采用氨基色谱柱,以乙腈-水为流动相梯度洗脱,用蒸发光散射检测器检测,在质量浓度为0.01～10.0 mg/mL内具有良好的线性关系,回收率在92.0％～ 103.5％,精密度(RSD)为2.98％ ～ 5.56％,检出限为0.04 mg/g.该方法可用于乳制品中乳果糖的日常监测.