通过菌种筛选选取最适制曲菌种,确定碳源添加量、种曲添加量、鱼体含水量、培养时间等单因素对制曲的影响,在此优化的基础上进行正交试验.经菌种筛选选用As3.042:宇佐美曲霉=3∶1制曲获得的蛋白酶酶活最高,制曲的最佳条件是葡萄糖添加量为3％,鱼体含水量为30％,制曲时间为48h,种曲添加量为2％.%Optimum strains for koji were screened and the effect of mold addition, glucose addition, water content of the fish and koji-making time were studied. Based on single-factor experiment, the koji-making conditions were optimized with orthogonal test. The research conclusion can be summarized as follows, the maximum protease activity occurred when ratios of mixed strains of As 3.042 and Aspergillus usamii were 3:1, and the optimum koji-making conditions were cultivation for 48h, raw material of 25g, glucose addition 3%, fish body water content 30%, and mold addition 2%.
展开▼
