蛹虫草菌静息细胞培养方法及纤溶酶的诱导研究

摘要

Using the fibrinolytic enzyme activity and biomass as evaluation indexes,resting cell culture method of Cordyceps militaris was established,and the induction of casein peptide on fibrinolytic enzyme was researched.The results showed that the carbon and nitrogen sources in resting cell culture medium were determined as glucose 0.08％ and urea 0.06％.The culture conditions were medium initial pH 7.0,inoculum 10％,shaker speed 180 r/min,culture temperature 23 ℃ and time 12 h.Under the conditions,the soluble circle area of plasmin in the fibrin plate was 34.97 mm2,the biomass of C.militaris was 2.38 g/L.The casein peptide component C2 was a direct inducer for fibrinolytic enzyme synthesis,and when the component C2 addition was 0.1 g/L,the activity of fibrinolytic enzyme was increased by 264％.%以纤溶酶活力和生物量为评价指标,建立了蛹虫草菌静息细胞培养方法,并研究了酪蛋白肽对纤溶酶的诱导作用.结果表明,静息细胞培养基中碳源和氮源确定为葡萄糖0.08％和尿素0.06％;培养条件为培养基初始pH 7.0,接种量10％,摇床转速180 r/min,23℃培养12h.在此条件下,纤溶酶在血纤维蛋白平板上形成的溶圈面积为34.97 mm2,蛹虫草菌生物量为2.38 g/L.酪蛋白肽组分C2是合成纤溶酶的直接诱导物,当其添加量为0.1 g/L时,纤溶酶活力较对照提高了264％.