纳米铜对大鼠肝脏毒性的蛋白质组2-DE图谱分析

摘要

In order to obtain protein expression map of nanoparticles copper(nano-copper) toxicity in rat liver, this study constructed the model of the nano-copper in rats, and obtained 2-DE maps of nano-copper toxicity in rats group and control group used by two-dimensional gel electrophoresis technique, PDQuest 8. 0 analysis showed that the total 43 protein spots significantly differently expressed in rat liver of 200 mg/kg copper nanoparticles group which 27 spots were up and 16 spots were down. The toxicity of nano-copper in rats liver was studied by proteomics approach in the paper, the results would establish the foundation for clarifying the mechanism of the toxic effects of nano-copper at protein level.%为获取纳米铜对大鼠肝脏毒性的蛋白质组表达图谱,试验通过构建大鼠纳米铜中毒模型,采用双向凝胶电泳技术获取了大鼠纳米铜中毒组和对照组肝脏的蛋白质2-DE图谱,结合PDQuest 8.0软件分析发现200 mg/kg纳米铜组大鼠肝脏2-DE图谱中有27个蛋白质斑点表达上调,16个蛋白质斑点表达下调,共有43个差异蛋白质点.试验利用蛋白质组学技术研究大鼠纳米铜肝脏蛋白质组学,为在蛋白质水平阐明纳米铜的毒性作用机制奠定基础.