lncLER在卢氏绿壳蛋鸡肝脏中表达调控的研究

摘要

Long noncording RNA (lncRNA) played an important regulating role in many biological processes.The lncRNA expression profile of liver of pre-laying hens (20 weeks old) and peak-laying hens (30 weeks old) in Lushi Green-shelled-egg chicken were obtained by lncRNA-seq.A lncRNA, which was named lncLER and expressed highly in the peak-laying hens was singled out.To investigate the expression characteristics of lncLER in liver of chicken,reverse-transcript PCR and Real-time fluorescence quantitative PCR were used to analyze the temporal expression profile of lncLER.Both individual chicken and primary hepatocytes were used to look into the effects of estradiol on expression of the lncLER in vivo and in vitro.The results showed that lncLER was expressed differently in various tissues and it's expression level was higher in the liver of peak-laying hens.The expression of lncLER, both in individual chicken and in primary hepatocytes,was up-regulated by estradiol.This research paved the way for further studying on the biological functions of lncLER in chicken egg production.%长链非编码RNA(lncRNA)在许多生物学过程中发挥着重要的调节作用.河南农业大学家禽种质资源创新工程研究中心通过对产蛋前期(20周龄)和产蛋高峰期(30周龄)卢氏绿壳蛋母鸡肝脏组织的转录组分析,筛选出了一个在产蛋高峰期母鸡肝脏中表达水平显著上调的lncRNA(lncLER).本研究利用半定量PCR(RT-PCR)、实时荧光定量PCR分析了lncLER在卢氏绿壳蛋母鸡不同发育时期和不同组织中的表达规律,并从个体和细胞水平研究了体内、体外雌激素处理对lncLER表达的影响.结果表明,lncLER在卢氏绿壳蛋母鸡各组织中均有不同程度的表达,且在产蛋高峰期肝脏中的表达量较高;雌激素在个体和细胞水平可显著上调lncLER的表达.本试验初步阐明了lncLER基因在卢氏绿壳蛋母鸡中的表达调控规律,为进一步研究lncLER在鸡产蛋过程中的生物学功能奠定了基础.