慢性冷热应激对三河牛血液生化指标及相关基因表达的影响

摘要

The purpose of this study was to detect biochemical indexes and genes related to cold and heat stress and non-stress in Sanhe cattle.Using real-time fluorescence quantitative PCR and ELISA,the expression quantity of HSP70 family genes (HSPA1A,HSPA1B,and HSPA8) mRNA in lymphocyte and concentrations of CK,LDH,GLU and BUN in serum were detected respectively after cold stress,heat stress and non-stress time.The results showed that CK and LDH activity in serum of cows were higher than non-stress time during cold and heat stress time,especially remarkably rose during heat stress (P＜0.05).The content of GLU was remarkably lower than non-stress time during heat stress time (P＜0.05).The content of BUN showed no remarkable changes during heat stress time.The content of GLU and BUN were remarkably higher than non-stress time during cold stress time (P＜0.05).Three HSP70 family genes mRNA expression quantities in lymphocyte of Sanhe cattle were increased during cold and heat stress.The results indicated that high and low temperature changed the activities of enzyme,glucose and protein metabolism in serum.Enhanced expressions of HSP70 family genes mRNA during cold and heat stress time improved the function of animal selfprotection and enhanced resistance to adverse external stimulate.%为研究冷热应激对三河牛血液生化指标及相关基因表达量的影响,利用实时荧光定量PCR和ELISA方法检测冷热应激期和非应激期血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)、葡萄糖(GLU)和尿素氮(BUN)含量及淋巴细胞中HSP70家族基因(HSPA1A、HSPA1B、HSPA8) mRNA表达量.结果显示:1)冷热应激期血清中CK和LDH活性相比非应激期均升高,热应激期升高尤为显著(P＜0.05);热应激期GLU含量相比非应激期显著降低(P＜0.05);热应激期BUN含量略低于非应激期,差异不显著;冷应激期GLU和BUN含量相比非应激期均显著升高(P＜0.05).2)冷热应激期淋巴细胞中3个HSP70家族基因mRNA表达量相比非应激期显著升高(P＜0.05).结果表明,慢性冷热应激改变了血液中酶的活性、糖和蛋白质的代谢,从而导致奶牛的一些组织细胞的损伤和营养代谢的紊乱,并冷热应激时血液淋巴细胞中HSP70家族基因mRNA表达量的上升,可在一定程度上反映其对动物机体的保护水平.