槲皮万寿菊素金属配合物的合成、抗氧化活性及与DNA结合的研究

摘要

Three kinds of Cu(II) ,Zn(II) ,Fe(lIl) complexes of quercetagetin were synthesized and characterized by IR spectra,UV- spectra and elemental analysis. The free radical (DPPH·,O-2·,·OH) scavenging activities of the complexes were investigated. The mechanism of the interaction between the free ligand and complexes with calf thymus DNA(ct-DNA) were investigated by means of UV spectra. The results showed that free radical scavenging activities of the complexes on DPPH·,O-2·and·OH were obviously stronger than that of the quercetagetin. Results of spectroscopic measurements suggested that the three complexes bound to DNA via an intercalative mode and calculated DNA-binding action decreased in the order Que-Fe>Que-Cu>Que-Zn> Que. The new metallic complexes of quercetagetin added new contents for coordination chemistry. The good bioactivity of the complexes afford an applied foundation on the medicine.%合成了槲皮万寿菊素铜(Ⅱ)、锌(Ⅱ)铁(Ⅲ)三种配合物,采用红外、紫外光谱及元素分析等方法分析了其配位情况,研究了配合物清除DPPH自由基、超氧阴离子自由基和羟基自由基的活性,并运用紫外光谱滴定实验,研究了配体及配合物与ct-DNA的相互作用.结果表明,配合物较配体具有更强的清除DPPH自由基、超氧阴离子自由基和羟基自由基活性；槲皮万寿菊素及其配合物以插入模式与DNA结合,与DNA结合作用的大小顺序为:Que-Fe＞Que-Cu＞Que-Zn＞ Que.