超快速高效液相色谱-三重四级杆串联质谱法测定水中微囊藻毒素的研究

摘要

A method of determination of microcystins in water by ultra fast high performance liquid chromatography/triple quadru-pole mass spectrometry was established in this paper. Water samples were directly filtrated and Mcs were seperated by UFLC-20A XR,analyzed by LC-MS8040. 5 Mcs had good linearity between 0. 10 μg·L-1 ~50 μg·L-1 . LODs are between 0. 0092 μg·L-1 ~0. 0743 μg·L-1 . LOQs are between 0. 0279 μg·L-1 ~0. 2253 μg·L-1 . The method is simple,rapid and highly sensitive,which can meet the requirements for the analysis of Mcs in water.%建立了一种利用超快速高效液相色谱-三重四级杆串联质谱仪测定水中5种微囊藻毒素的方法。样品经滤膜过滤后，利用超快速高效液相色谱UFLC-20A XR进行分离，三重四级杆串联质谱LCMS-8040外标法进行定量分析。5种微囊藻毒素RR、YR、LR、LY、LF在0．1~50μg·L-1浓度范围内线性良好，定性检出限在0．0092μg·L-1~0．0743μg·L-1之间，定量限在0．0279μg·L-1~0．2253μg·L-1之间，加标回收率分别在85．4%~108．0%之间。该方法简便、快捷，适用于水中微囊藻毒素的测定。