凝集素修饰的Fe2O3@Au纳米粒子与SW620细胞的相互作用研究

摘要

首先采用共沉淀法及交互盐酸羟胺还原法制备Fe2O3@Au核壳结构纳米粒子,利用凝集素修饰纳米粒子(Lectin-Fe2O3@Au NP),然后通过动态光散射(DLS)/聚丙烯酰胺凝胶电泳/磁滞回曲线进行表征.利用MTT法测纳米粒子的细胞毒性,将不同浓度的Lectin-Fe2O3@Au与结直肠癌SW620细胞相互作用,普鲁士蓝进行染色,使用紫外可见光谱法(UV-Vis)和光学显微镜进行观察.结果表明凝集素修饰的纳米粒子在细胞培养基中能够稳定存在,当纳米粒子浓度为0.72 nM时,结直肠癌SW620细胞的存活率仍高于90%,其作用强度依次为蓖麻凝集素(RCA)-Fe2O3@Au>麦胚凝集素(WGA)-Fe2O3@Au>伴刀豆素(ConA)-Fe2O3@Au,经过普鲁士蓝染色的细胞可以观察到RCA-Fe2O3@Au纳米粒子的存在,表明凝集素RCA具有作为修饰纳米粒子与结直肠癌SW620细胞靶向识别的潜力.%Fe2O3@Au core-shell nanoparticles were successfully prepared by co precipitation method and the interaction of hydrox-ylamine hydrochloride. Further nanoparticles were modified by lectin,the structure and physicochemical characterizations were proved by dynamic light scattering(DLS)/polyacrylamide gel electrophoresis/magnetization curve. The cytotoxicity of the lectin-conjugated Fe2O3@Au nanoparticles was evaluated through MTT cell proliferation assays,and dyed by Prussian blue.the interac-tions of three lectin-conjugated Fe2O3@Au nanoparticles with colon cancer cell line SW620 were studied by optical ultraviolet-visi-ble spectrometer and microscope.The results showed Lectin-Fe2O3@Au nanoparticles had highly salt stability and stable in cell culture medium.The survival rate of colorectal cancer SW620 cells was still higher than 90%when the concentration of nanoparti-cles was 0.72 nM,the binding affinity with SW620 was(Ricinus Communis Agglutinin)RCA-Fe2O3@Au>(Wheat Germ Aggluti-nin)WGA-Fe2O3@Au>(Concanavalin)ConA-Fe2O3@Au,the existence of RCA-Fe2O3@Au nanoparticles could be observed in the cells which were dyed by the Prussian blue.These findings suggested that the RCA-Fe2O3@Au could be potentially employed as a target agent in the recognition of colon cancer cell SW620.