表面等离子体共振技术用于蛋白对氨基酸手性识别的动力学研究

摘要

Based on surface plasmon resonance(SPR) technique, a new method was developed for efficiently recognizing the chiral discrimination between the small molecule L- and D-amino acid enantiomers without any labeling. Bovine serum albumin and lysozyme were selected as chiral recognition probes, and phenylalanine and tryptophan including four L-, D-enantiomers were used as analytes. While six different concentrations of each amino acid enantiomers interacted with two protein probes at the same time, the binding kinetics informations of them were obtained in real time. The results showed that proteins had excellent chiral recognition capability to L- and D-enantiomers. The chiral discrimination between each of amino acid enantiomers could be recognized obviously by comparising either their binding kinetics parameters or sensorgram. The results also indicated that the affinity of protein and L-amino acid was bigger than that of the protein and D-amino acid.%利用表面等离子体共振(SPR)技术,以牛血清白蛋白和溶菌酶为探针构筑了手性识别传感膜,对L-和D-苯丙氨酸以及L-和D-色氨酸手性识别的动力学进行了研究.结果表明,两种蛋白在与每种氨基酸分子的L和D型异构体相互作用过程中都存在明显的动力学差异.动力学数据显示,两种蛋白与每种氨基酸L型异构体的亲和力均大于D型.