A new surface plasmon resonance imaging (SPRi) system was designed and fabricated using a right angle trapezoid prism as SPR coupler, which allows the fixation of left optical elements in a compact space and addition of a 1-2x zooming adjuster. This design simplifies the angle scanning mechanism, enables a very fine adjustment of incident angle from 40° to 80° at 0. 001° angle resolution, and makes the observation possible of size-variable chips( 1-4 cm2) , providing a lateral spatial resolution up to 3600 Point/cm2. Its sensitivity was evaluated with sucrose solutions, giving the data up to 7xl0-6 RIU(Refractive index unit). A linear relationship was obtained between resonance angle and sample refractive index, with correlation coefficients of 0. 99953. The new system has been further validated in the immuno-reaction of BSA with anti-BSA, which enables to produce both of kinetic reaction curves and differentiated dot images. These results indicate that the newly designed SPRi system is highly promising for high-through binding affinity applications.%设计了一种折反式棱镜耦合单元,用于简化表面等离子共振系统的角度调节操作,并基于此单元构建了固定光路可变焦宽调角表面等离子共振成像装置.该装置通过旋转折反式棱镜耦合单元即可实现角度调节,调节范围可达40°～80°,结合1～2倍的变焦成像系统,可对1～4 cm2传感芯片成像,且可分辨至少3600 Point/cm2.以蔗糖溶液为研究对象,考察了该装置的灵敏度,结果表明其灵敏度可达7×10-6 RIU( Refractive index unit),共振角与折射率的线性相关系数为0.99953.将该装置用于研究抗牛血清白蛋白抗体与其它蛋白的实时相互作用,所得结果与理论相符.该表面等离子共振成像装置将在实时并行分析方面具有广阔的应用前景.
展开▼
