β-catenin与小鼠毛囊衰老性变化相关

摘要

Objective To investigate the relationship between p-catenin and mice hair follicle senescence. Methods The expression pattern of β-catenin in hair follicles of two age groups of C57 mice was eexamined by immuno-fluorescence, RT-PCR, and Western blot. Results Compaired to young mice, both β-catenin mRNA and protein expression levels were upregulated in old mice hair follicle at various stages of hair cycle. In the anagen skin of 34months old mice, β-catenin protein expressions were at the level of 0. 9024 ±0. 0167, significantly higher then the level in the anagen skin of 35 days old mice(0. 8012 ±0. 0184). In the telogen skin of 34months old mice, β-catenin protein expressions were 0. 6348 ±0. 0241 , significantly higher than the that in the telogen skin of 23 days old mice ( 0. 6348 ± 0. 0241 ) ( P < 0. 05 ) . β-catenin protein distribution was localized at the up-portion of outer root-sheath and Bulge cells, while the nuclear expression was obviours. No apparente difference of the p-catenin expression pattern was observed in various stages of hair cycle in senile mice. Conclusion In old mice hair follicle, the changes of β-catenin expression pattern including the expression level, periodicity and localization may contribute to the senescent poliosis.%目的 探讨β-catenin与毛囊衰老性变化的关系.方法 取老龄与年轻小鼠处于毛囊周期各阶段的皮肤,RT-PCR和Western blot检测β-catenin在mRNA和蛋白水平的表达;IF检测比较β-catenin蛋白表达在毛囊中的组织学分布.结果 老年鼠背皮的β-caternin表达相较处于同一毛囊周期阶段的年轻鼠皮肤在mRNA水平和蛋白质水平均有上调,出生后34个月的小鼠生长期皮肤中β-catenin蛋白的表达为0.9024±0.0167,显著高于出生后35d的0.8012±0.0184.出生后34个月的小鼠静止期皮肤中β-catenin蛋白表达为0.6348±0.0241,显著高于出生后23d的0.6348±0.0241(P＜0.05).IF检测显示β-catenin的表达集中在外根鞘和Bulge细胞,也可以观察到β-catenin的入核现象.结论 β-catenin表达量上调,周期节律不明显,表达部位不同于年轻鼠可能是衰老相关性白发产生的原因之一.