血清SCCA水平及盆腔淋巴结HPV16/18感染状况与宫颈鳞癌患者预后的关系

摘要

Objective To explore the relation of preoperative serum SCCA levels, pelvic lymph node HPV 16/18 infection in patients with cervical squamous cell carcinoma and their prognosis. Methods ELISA was performed to detect the SCCA level and real-time fluorescence quantitative PCR was utilized to analyze the positive rate of pelvic lymph node infection with HPV 16/18. The relation of SCCA level, HPV 16/18 positive rate and the three-year survival rate was investigated. Results The SCCA level was closely related with the infection of HPV 16/18 in pelvic lymph node ( P < 0. 05 ). The mean follow-up time for 35 patients with cervical squamous cell carcinoma was 36 m (rang 12~41 months). Four patients died and 3 year survival rate was 85. 0%. The 3 year survival rates for patients with SCCA positive and negative were 100% and 69. 2% respectively and that were 96. 4% and 57. 1% for patients with positive or negative pelvic lymph node infection with HPV 16/18. The 3-year survival rate for patients rnwith negative SCCA and lymph node HPV 16/18 infection were obviously longer than that of positive ones (P <0. 05) . lymph node metastasis, SCCA level and pelvic lymph node HPV 16/18 infection status were associated with prognosis. Conclusions SCCA level and pelvic lymph node HPV 16/18 infection are associated with the prognosis of cervical squamous carcinoma patients and is a potential markers for cervical squamous carcinoma.%目的 探讨术前血清SCCA水平、盆腔淋巴结HPV16/18感染状况与宫颈鳞癌患者预后的关系.方法 ELISA法检测血清SCCA水平,实时荧光定量PCR法检测盆腔淋巴结HPV16/18感染,分析二者与宫颈鳞癌患者3年生存率的关系.结果 血清SCCA水平与盆腔淋巴结HPV16/18感染相关(P＜0.05).35例宫颈鳞癌患者中位随访36个月(12～41月),死亡4例,3年总体生存率为85.0％.血清SCCA阴性者和阳性者的3年生存率分别为100％和69.2％;盆腔淋巴结HPV16/18阴性者和阳性者的3年生存率分别为96.4％和57.1％;血清SCCA及盆腔淋巴结HPV16/18阴性患者的3年生存率明显优于阳性者(P＜0.05).淋巴结转移、血清SCCA和淋巴结HPV16/18与宫颈鳞癌患者的预后相关.结论 血清SCCA水平及盆腔淋巴结HPV16/18感染状况与宫颈鳞癌患者预后相关,可作为宫颈鳞癌预后判断指标.