ChIP在研究DNA与蛋白质相互作用中的应用

摘要

The interaction between DNA and protein is one important field of gene expression regulation in the post-genome era. There are many methods to study the interaction between DNA and protein at present, but chromatin immunoprecipitation assay (CHIP) is more suitable comparing with them. Besides, cloning technique and DNA chip technology could be combined to high-throughput screen the unknown DNA targets of known protein factors and study the distribution of trans-acting factors in whole genome so as to enhance the development and occurrence of DNA-protein interaction regulatory networks. The research progress of ChIP was introduced, and its prospect was prospected.%在后基因组时代,基因表达调控的一个重要领域就是DNA-蛋白质的相互作用。目前存在多种研究DNA-蛋白质的方法,但是这些方法与染色质免疫沉淀技术（ChIP）相对比而言,染色质免疫沉淀技术更加适合研究DNA-蛋白质的相互作用,因此是一种较理想的研究方法。另外,由于存在克隆技术与DNA芯片技术,因此可使之充分结合以用于高通量筛选已知蛋白因子的未知DNA靶点和研究反式作用因子在整个基因组上的分布情况,以促进DNA-蛋白质相互作用调控网络的发展与出现。对ChIP技术进行了介绍,概述了该技术的研究进展,并就其前景进行了展望。