Aim To develop an optimal RP-HPLC method for determining the content of mesaconitine and hypaconitine in the aerial part of Radix Aconiti Lateralis Praeparata. Methods Two constituents were analyzed simultaneously with a Venusil ABS C18 column (250 mm × 4.6 mm, 5 μm) by gradient elution using acetonitrile-0. 1％ ethylenediamine as the mobile phase. The column was maintained at 30 ℃ . The flow rate was 1 ml . min -1. The detection wavelength was set at 230 nm. Results Excellent chromatographic separation was achieved for mesaconitine and hypaconitine. The linear ranges of the two constituents were 12. 5 ～ 1 200 mg · L-1 , and 11. 46 ～ 100 mg ～ L-1 respectivety. The extraction recoveries were 99. 72％ ( RSD = 0. 69％ ), and 100. 29％ ( RSD = 1. 07％ ) . Conclusion The method has the advantages of simplicity, acuteness and precision,which is suitable for quantitative analysis of mesaconitine, hypaconitine in Radix Aconiti Lateralis Praeparata.%目的 建立RP-HPLC法,测定附子地上部分中新乌头碱和次乌头碱的含量.方法 采用Venusil ABS C18色谱柱(250 mm × 4.6 mm,5 μm),以乙腈-0.1% 乙二胺溶液为流动相,梯度洗脱,柱温30 ℃,流速 1 ml·min-1,检测波长230 nm.结果 新乌头碱、次乌头碱均得到较好分离,分别在12.5～1 200 mg·L-1、11.46～1 100 mg·L-1呈良好的线性关系;加样回收率分别为99.72%(RSD=0.69%),100.29%(RSD=1.07%).结论 该方法简便、灵敏、准确,可用于附子中双酯型生物碱的定量分析.
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