高效液相色谱法测定马兰中大黄素的含量

摘要

目的 建立测定中草药马兰中大黄素含量的高效液相色谱方法.方法 甲醇回流提取药材,高效液相色谱法测定,采用的色谱柱为Kromasil-C18柱(250 mm×4.6 mm,5 μm);流动相为甲醇-0.1%磷酸水溶液(80:20);检测波长为254 nm;柱温为30℃;流速为1 mL·min-1.结果 大黄素溶液在1.4～14 mg·L-1浓度范围内与其峰面积呈良好线性关系(r=0.999 3),平均加样回收率为99.61%,其RSD值为1.08%(n=6).结论 该方法准确、可靠、灵敏、简便,适合中草药马兰中大黄素含量的测定.%Objective To establish an HPLC method for determination of emodin in Kalimeris indicai L. )Sch-Bip. Methods The dry medicinal herb was extracted by refluxing in methanol and determined by HPLC. A Kromasil-C18 column was used with the mobile phase composed of methanol-phosphoric acid solution( 0. 1% )( 80:20 ) at the flow rate of 1 mL · min-1 . The detection wavelength was 254 nm and the column temperature was 30 ℃. Results Emodin showed a good linear relationship to the peak aera at a concentration range of 1.4-14 mg · L-1( r =0.999 3 ). The average recovery was 99. 16% with RSD of 1.08%( n =6 ). Conclusion The method is accurate, reliable, sensitive, simple and suitable for the determination of emodin in Kalimeris indicai L. )Sch-Bip.