基于Diurnal在线软件分析拟南芥生物节律基因对GABA的响应

摘要

Diurnal是一个基于网络的在线分析工具，利用该工具可以得到几种模式植物基因组范围内的昼夜和生物钟节律基因表达的变化规律。这一在线分析软件是一个可供搜索的数据库，它提供了几个基于网络的界面友好的数据挖掘工具，其结果以一种易于理解的方式呈现。在该研究中首先介绍了这个在线软件的使用方法，然后分析了几个拟南芥生物钟节律基因和开花基因在长日照条件下的表达模型，这些结果可以以图形和数字的方式下载用于后续的特定研究。结合在线数据，该研究表明，γ-氨基丁酸（GABA在1.0mmol/L）可以改变3个生物钟核心节律基因（TOC1，CCA1和LHY）的表达模式，这种模式的改变主要是增加了这些基因表达的幅度。%Diurnal is a web-based tool for accessing the diurnal and circadian genome-wide expression re-sults of genes from several array experiments conducted on common model plants. The database is search-able and provides access to several user-friendly Web-based data-mining tools with easy-to-understand output. Here we first introduced the method of this online software application and analyzed the model pat-tern of some circadian clock gene components in the longday growth condition in Arabidopsis. These re-sults can be accessed both graphically and numerically downloaded for further use in a specific study. Combining with the wet-lab experiment,we showed that application of γ-aminobutyric acid(GABA at 1.0 mmol/L)can change the pattern of the 3 core circadian clock gene(TOC1,CCA1 and LHY),mainly increas-ing the amplitude of the gene expression.