NGF和CgA对RWPE-1细胞内钙离子浓度变化的影响及其在慢性前列腺炎中的作用

摘要

Objective To explore difference of intracellular calcium ion ( [ Ca2+] i ) concentration of nerve growth factor( NGF) and chromogranin A( CgA) treated prostate epithelial cell line RWPE-1 cells and evaluate the role of NGF and CgA in chronic prostatitis (CP). Methods Using different concentrations of NGF and CgA (50 ng/ml and 200 ng/ml) to stimulate RWPE-1 cells, the difference of intracellular [ Ca2+] i concentration was observed by using Laser Scanning Confocal Microscopy. The difference of intracellular[ Ca2+] i concentration was observed after infusing NGF and CgA mixed with TRPV1 receptor antagonist. When the extracellular solution was changed to D-PBS, the intracellular [ Ca2+] i concentration was observed after NGF and CgA infusion. Results Change in intra-cellular [ Ca2+] i concentration did not occur by infusing 50 ng/ml NGF and CgA. Intracellular [ Ca2+] i concentra-tion significantly increased by infusing 200 ng/ml NGF and CgA. Intracellular [ Ca2+] i concentration did not change after infusion of NGF and CgA mixed with TRPV1 receptor antagonist. Even when the extracellular solution was changed to D-PBS, the intracellular [ Ca2+] i concentration did not change after NGF and CgA infusion. Con-clusion NGF and CgA might participate in the mechanism of CP and cause the opening of TRPV1 of prostate epi-thelial cell membrane. Then, the intracellular [ Ca2+] i concentration increase. In the end, prostate epithelial se-cretion decline.%目的 探讨神经生长因子(NGF)和嗜铬颗粒蛋白A(CgA)处理后前列腺上皮细胞系RWPE-1细胞内钙离子浓度([Ca2+]i)的差异及NGF和CgA在慢性前列腺炎(CP)发病中的作用.方法 不同浓度的NGF和CgA(50、200 ng/ml)刺激RWPE-1细胞,用激光共聚焦显微镜观察不同浓度的NGF和CgA刺激后细胞内[Ca2+]i变化,加入TRPV1受体拮抗剂后继续观察细胞内[Ca2+]i变化.然后,将正常细胞外液换成D-PBS,再加入NGF和CgA观察细胞内[Ca2+]i变化.结果 50 ng/ml NGF和CgA刺激细胞后,细胞内[Ca2+]i未见明显变化.200 ng/ml NGF和CgA刺激细胞后,细胞内[Ca2+]i明显增加,加入TRPV1受体拮抗剂后,细胞内[Ca2+]i未见明显变化.将正常细胞外液换成D-PBS,用NGF和CgA刺激后,细胞内[Ca2+]i未见明显改变.结论CP的发生可能是通过分泌和释放NGF和CgA,引起前列腺上皮细胞膜TRPV1通道开放,促使细胞内钙离子浓度增加,导致前列腺上皮分泌功能下降所致.