叉状头/翅膀状螺旋转录因子在BXSB狼疮小鼠肾脏中的表达

摘要

目的 观察叉状头/翅膀状螺旋转录因子(Foxp3)在BXSB狼疮小鼠肾脏中的表达,探讨Foxp3在狼疮性肾炎中可能的免疫耐受诱导作用及机制.方法 选取8周龄及16周龄雄性BXSB小鼠各6只,以8周龄C57BL/6雄性小鼠6只为正常对照,用免疫组织化学及实时荧光定量PCR方法,检测Foxp3在小鼠肾脏中的表达.结果 正常对照组C57BL/6小鼠肾组织Foxp3阳性表达,细胞呈深棕色着色,广泛分布于肾小管;8周龄及16 周龄BXSB小鼠肾脏组织Foxp3弱阳性表达,细胞呈浅棕色着色,分布于肾小管.8、16周龄BXSB组小鼠肾组织内Foxp3 mRNA表达水平[(0.55±0.06),(0.51±0.07)]均较正常对照组(1.02 ±0.08)降低,差异有统计学意义(均P＜0.01);16周龄BXSB组小鼠的Foxp3 mRNA表达水平较8周龄BXSB组降低,但差异没有统计学意义(P＞0.05).结论 BXSB小鼠狼疮肾炎的发病机制可能与调节性T细胞的Foxp3表达下调相关.%Objective To investigate the expression of forkhead/winged helix transcription factor (Foxp3) in the kidney of BXSB mice and its potential role in inducing the immune tolerance pathogenesis of lupus nephritis in BXSB mice. Methods Foxp3 was detected in controls,8 week BXSB male mice and 16 week BXSB male mice by using immunohistochemistry and real time PCR. Results Foxp3 protein staining in control group was strong as dark brown and distributed in renal tubules,but in 8 and 16 week BXSB mice the staining was weaker as light brown than controls- The mRNA expression of Foxp3 was significant ly decreased in renal tissues of 8 and 16 week BXSB mice as compared with that in the controls[(0. 55 + 0. 06) and (0. 51 + 0. 07) vs (1. 02 + 0. 08) ,P<0. 01],but there was no significant difference between 8 and 16 week BXSB mice(P>0. 05). Conclusion Down regulation of Foxp3 in regulatory T cells may be involved in the pathogenesis of lupus nephritis in BXSB mice.