Objective To observe the emotional response to inflammatory pain model induced by complete Fre⁃und’ s adjuvant ( CFA) , and to explore the relationship between phosphate kinase C ( PKC) zeta in the anterior cingulate cortex ( ACC) and emotional response to inflammatory pain. Methods Twenty⁃four healthy 2⁃month old female Sprague⁃Dawley rats were randomly divided into control group and model group. The inflammatory pain model was established by in⁃jecting subcutaneously with 0�1 ml CFA into the right hind paw of rats. To observe the dynamical changes of body weight and pain threshold before modeling, and at 3, 7, 14, 21, and 28 days after modeling. Detecting the total distance, dis⁃tance in open arm, the number of times entering into the open arm, percentage of time spent in open arm of elevated O maze were measured at 14, 21, 28 days after modeling. The total distance, distance in the central quadrat, the number of times entering the central quadrat, and time spent in the central quadrat of open field were determined at 14 and 29 days af⁃ter modeling. The protein expression of PKCzeta in the ACC was detected by Western blot at 14 and 29 days after CFA⁃in⁃jection. Results There were no significant differences of body weight between the control and model groups (P>0�05). Compared with the control group, the pain threshold of rats in the model group was significantly decreased at 1, 3, 7, 14, 21, and 28 days after CFA⁃injection (P<0�05), the distance in open arm and percentage of time spent in open arm of the model group were markedly reduced at 28 days after modeling (P<0�05), distance in the central quadrat and the number of times entering into the central quadrat of model group were significantly decreased at 29 days after modeling (P<0�05), and the protein expression of PKCzeta in ipsilateral ACC was increased at 29 days after CFA⁃injection (P<0�05). There was a negative correlation between distance in open arm and protein expression of PKCzeta in the ipsilateral ACC, percent⁃age of time spent in open arm and protein expression of PKCzeta in the ipsilateral ACC, distance in the central quadrat and protein expression of PKCzeta in ipsilateral ACC, the number of times entering into the central quadrant and protein expres⁃sion of PKCzeta in the ipsilateral ACC, respectively. Conclusions Abnormal emotional behavior may occur in rats with chronic inflammation pain induced by CFA⁃injection. This pain emotion behavior may be associated with high protein ex⁃pression of PKCzeta in the ipsilateral anterior cingulate cortex.%目的：观察CFA致炎性痛模型大鼠的情绪反应，探讨前扣带皮层（ ACC）磷酸激酶 Czeta（ PKC⁃zeta）与炎性痛大鼠情绪反应的关系。方法24只清洁级雄性 SD大鼠随机分为空白对照组和模型对照组。足底皮下注射弗氏完全佐剂建立慢性炎性痛模型。动态观察各组大鼠造模前（ base）、造模后3、7、14、21和28 d的体重和痛阈变化；观察造模后14、21、28 d所有大鼠在高架O迷宫中的总运动距离、开放臂运动距离、开放臂进入次数和开放臂停留时间百分比。观察造模后14、29 d所有大鼠在旷场中的总运动距离、中央象限运动距离、中央象限进入次数和中央象限停留时间。采用免疫印迹法检测造模后14 d和29 d健侧和患侧ACC区域PKCzeta蛋白表达。结果各个时间点两组大鼠体重差异无显著性（ P＞0�05）。造模前，两组大鼠痛阈差异无显著性（P＞0�05）；造模后1 d，模型对照组大鼠痛阈显著降低（P＜0�05），且在整个实验过程中均显著低于空白对照组（P＜0�05）。与空白对照组比较，模型对照组大鼠于模后28 d开放臂运动距离和开放臂停留时间百分比显著减少（P＜0�05），于模后29 d中央象限运动距离和中央象限进入次数明显减少（P＜0�05）。造模后29 d，模型对照组大鼠患侧ACC区域 PKCzeta蛋白表达明显多于空白对照组（P＜0�05）。且开放臂运动距离、开放臂停留时间百分比、中央象限运动距离、中央象限进入次数与患侧 PKCzeta蛋白表达变化呈负相关。结论 CFA诱导的慢性炎性痛大鼠可出现异常情绪行为；慢性炎性痛情绪样行为可能与ACC区域PKCzeta的高表达相关。
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