Three materials (I:25% sandy soil+75% wheat field soil;Ⅱ:25% wheat field soil+75% spent Pleurotus ostreatus substrate containing 89% cottonseed hulls、10% wheat bran and 1% lime;Ⅲ:grassland soil)were used as casing soils in Coprinus comatus cultivation.Selected physicochemical properties and viable bacterial counts of the casing soils were determined,and the effect of the different materials on the fruiting of Coprinus comatus was evaluated.Grassland soil (sample Ⅲ)exhibited the highest water holding capacity (73.4%)and porosity (7.3%)values.The original pH values of the three samples differed considerably but samples II and III were adjusted to pH 7.9 with lime prior to casing.Populations of viable bacteria in the casing soils increased rapidly after application until the appearance of the first flush of mushrooms (21 days), after which (21~30 d)the numbers decreased rapidly.A positive correlation was observed between the mushroom yield,water holding capacity,porosity and the size of the bacterial population.Overall,casing soil sample Ⅲ provided the highest fruiting levels.%分别以麦田土(含25%细沙)、菌糠土(麦田土25%、糙皮侧耳菌糠75%)、草炭土(100%)为覆土对毛头鬼伞(Coprinus comatus)进行出菇栽培,对出菇产量及生物学效率测定计算,并对覆土理化性状及其中可培养细菌生长量进行检测。结果表明：以草炭土作覆土子实体产量最高(606.4 g/kg),生物学效率达133.4%；草炭土孔隙度为7.3%、持水率为73.4%,该覆土中可分离培养细菌量在覆土21 d 最高达30×108 cfu/g,均明显优于另两种覆土基质。三种覆土中细菌量从覆土初始至首潮菇出土现蕾(覆土21 d)皆表现迅速增长,在菇蕾形成及出土后至采收期(覆土21~30 d),细菌数量却迅速减少。毛头鬼伞产量与三种覆土的孔隙度、持水率(含水量)、可培养细菌量(出土现蕾时)呈正相关。
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