To synthesize the molecular imprinted polymer ( MIP) for clenbuterol and study the recognition property of the MIP, polymethylacrylic acid was grafted onto the surface of silica gel particles via the coupling effect of γ-Methacryloxypropyl trimethoxy silane, and the grafting particle was prepared. Then, the molecular imprinting towards the grafted particle was performed with clenbuterol as template molecule and ethylene glycol diglycidylether as crosslinking agent by using the surface-molecular imprinting technique established, and the clenbuterol molecule-imprinted material was prepared. The morphology of MIP was observed by scanning electron microscope ( SEM ) , and the chemical structure of MIP was characterized by Fourier transform infrared spectroscopy (FT-IR). Static method was adopted to study the binding properties and molecule recognition character of MIP for clenbuterol. The experiment results showed that MIP had specific recognition selectivity, excellent binding affinity and elution property for clenbuterol.%为研究克仑特罗分子印迹材料的合成及识别特性,以3-(异丁烯酰氧)丙基三甲氧基硅烷为媒介,将聚甲基丙烯酸偶合接枝到硅胶微粒表面,形成接枝微粒,以克仑特罗为模板分子,乙二醇二缩水甘油醚为交联剂,对接枝在硅胶表面的聚甲基丙烯酸大分子链进行分子印迹,制备克仑特罗表面分子印迹材料.采用扫描电镜观察分子印迹材料表面,用红外光谱对分子印迹材料的化学结构进行了表征,采用静态法研究克仑特罗表面分子印迹材料对克仑特罗的结合性能与分子识别特性.研究表明,分子印迹材料对克仑特罗具有特异的识别选择性,优良的结合亲和性及洗脱性.
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