An aroma-producing strain GXY35 was isolated from 14 kinds of tobacco leaves, and the aroma components were analyzed by gas chromatography-mass spectrometry (GC/MS). The strain was identified as Klebsiella sp. from physiological and biochemical characteristics and 16S rDNA. The content of 4-vinylgualacol in fermentation broth was the highest. On the basis of single factor test, the fermentation condition of strain was optimized by orthogonal experi-ment. The optimal fermentation condition was as follows:the solid-liquid ratio of 1∶20, the inoculums size of 12%, the fermentation temperature of 37℃, the fermentation time of 72 h, and the initial pH of 7. 0. Under the optimal fermen-tation condition, the content of the characteristic favoring substance of 4-vinylgualacol was 0. 725 mg·mL-1 .%从14种烟叶表面筛选出一株能够利用烟末发酵产香的菌株GXY35,通过生理生化实验和16S rDNA序列对其进行分析,并用GC/MS对发酵液的香气成分进行分析。结果表明：GXY35分离株属于克雷伯氏杆菌,发酵液中特征香味物质4-乙烯基愈创木酚的含量最高。在单因素试验的基础上通过正交试验,对菌株的发酵条件进行优化。结果表明,最适宜的产香发酵条件为：料液比1∶20,接种量12%,发酵温度37℃,发酵时间72 h,pH值7．0。在此条件下,特征香味物质4-乙烯基愈创木酚的含量最高可达0．725 mg·mL-1。
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