甘肃黑猪H-FABP基因克隆、SNPs筛查及生物信息学分析

摘要

This study uses bioinformatics method to analyze the function and property of H-FABP gene encoding pro-tein of Gansu black pig. DNA pooling and direct sequencing techniques were used for SNPs rapid screening and the function prediction of H-FABP protein of Gansu black pig. The results showed that the CDS region of H-FABP gene in Gansu black pig was 402 bp,encoding 133 amino residues,4 SNPs in CDS region including C734T-Intron1,T152C-Exon2,G30A-Intron2 and T121G-Intron2. Among them, T152C-Exon2 was a missense mutation, and the amino acids were converted from threonine(Ile)to threonine(Thr). The bioinformatics prediction showed that H-FABP protein of Gansu black pig was a stable hydrophilic protein, and the secondary structure and tertiary structure were mixed protein mainly with β-fold.%采用生物信息学方法从分子水平对甘肃黑猪H-FABP基因编码蛋白质的功能和性质进行分析.采用DNA池和直接测序技术对甘肃黑猪H-FABP基因进行SNPs快速筛查及其编码区蛋白质功能预测.结果表明,甘肃黑猪H-FABP基因编码区全长402 bp,编码133个氨基酸残基,共检测出4个SNPs,分别为C734T-Intron1、T152C-Exon2、G30A-Intron2、T121G-Intron2,其中 T152C-Exon2为错义突变,氨基酸由原来的异亮氨酸(Ile)转变为苏氨酸(Thr).生物信息学预测发现,甘肃黑猪H-FABP蛋白是一种稳定的亲水蛋白,蛋白质二三级结构均以β-折叠为主的混合型蛋白.