多花黄精实生苗组培快繁技术研究

摘要

The effects of seed pretreatment methods, explant disinfection and sterilization methods, and different concentra-tions of plant hormone on the tissue culture and rapid propagation of Polygonatum cyrtonema seedlings were studied. The results showed that the best seed pretreatment way was directly getting out the seeds of P. cyrtonema from cold storage and then abrasing their seed coat;adopting this way could enhance seed germination rate and shorten germination time. The best explant disinfection and sterilization method was using 2% NaClO for 15 min first and then using 0.1% HgCl2for 14 min,which could obtain the low-est infection rate and the highest seed germination rate. The best culture medium for bud differentiation induction and proliferation was MS+6-BA 1.0 mg/L+GA30.2 mg/L. The optimum rooting medium was MS+NAA 0.3 mg/L, and the rooting rate reached 85%.%以多花黄精的种子为试材,开展种皮的不同预处理、不同外殖体的消毒灭菌方法以及添加不同浓度植物激素的实验,研究多花黄精组培实生苗生长的影响因子.结果表明:将黄精种子直接从冷库中取出后磨去种皮是发芽率较高,并能缩短发芽时间的较适种子预处理方式;使用2% NaClO消毒15 min后使用0.1% HgCl2消毒14 min是污染率较低且发芽率最高的消毒灭菌处理方式;最理想的芽分化诱导及增殖培养基为MS+6-BA 1.0 mg/L+GA30.2 mg/L;最理想的生根培养基为MS+NAA 0.3 mg/L,生根率高达85%.