Objective： To investgate the expression of chemotactic factor CCL20 in murinecollagen-induced arthritis(CIA)and the therapeutic effect of anti-CCL20 therapy on CIA,and to explore its potential value in the treatment of rheumatoid arthritis(RA).Method：s ①The model of CIA was established, forty DBA/1 murines wererandomly divided into four groups:healthy control group,CIA model group,CCL20monoclonal antibody group,tripterygium wilfordii group.②Joint swelling degree ofmurines on different time points was observed.③The pathological changes were evaluated,and the serum tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)level werealso measured.④The pathological changes in four groups were analyzed by the statisticalSPSS17.0.Result ：①Comparing to that of control group(2.83±1.37ng/L),the serum CCL20level in CIA model group(29.45±4.92ng/L)was increased significantly(P＜0.05).②theserum CCL20 level in CIA model group correlates with TNF-α and IL-1β;③Comparedwith CIA model group,both CCL20 monoclonal antibody group and tripterygium wilfordiigroup got improvement in general condition,joint swelling,inflammation cell infiltrationsynoviocyte proliferation,synoviocyte apoptosis,serum level of TNF-α and IL-1β(P＜0.05),the serum level of TNF-α and IL-1β in CIA model group were(63.1±282)ng/L,(83.72±4.04)ng/L respectively, tripterygium wilfordii group:(43.15±2.31)ng/L,(53.78±5.89)ng/L,CCL20 monoclonal antibody group:(40.91±2.02)ng/L,(56.77±4.98)ng/L,The two treatment groups compared with the model group,the difference wasstatistically significant(P＜0.05), ④In the pathological changes,there were nosignificant difference between CCL20 monoclonal antibody group and tripterygiumwilfordii group(P＞0.05).Conclusions: CCL20 is up-regulated expressioned in CIA murine,and it correlated withTNF-α and IL-1β;blocking the expressions by CCL20 monoclonal antibody caneffectively relieve the joint inflammation,and its effect of treatment is similar totripterygium wilfordii.CCL20 monoclonal antibody has potential value in treatment ofRA.
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