Effect of chrysin isolated from Oroxylum indicum against cisplatin-induced acute renal failure

摘要

Present study is designed to evaluate the effect of chrysin isolated from Oroxylum indicum against on cisplatin-induced nephrotoxicity. chrysin was isolated from dried roots of Oroxylum indicum. Nephroprotector activity was evaluated in male Albino rats. Nephrotoxicity induced by intraperitoneal administration (6 mg/kg,i.p., single dose) of cisplatin. Chrysin was administered by gastric intubation at two dose levels. Animals were divided into 5 groups. Group I animals received vehicle (1% CMC). Group II animals received cisplatin on day 1. Group III received 40mg/kg Bd.Wt chrysin for ten days, Group IV and V received 20 mg/kg and 40mg/kg Bd.Wt. chrysin respectively from day 1 to day 10 and On day 5 these groups received cisplatin injection. On day 9, urine was collected and estimated urinary functional parameters. Blood was collected on day 10 and estimated Blood urine nitrogen (BUN), Serum creatinine (SC). Malondialdehyde levels were also estimated in kidney tissue. Histological studies were also conducted. Cisplatin caused acute renal damage characterized by elevation of Blood Urine Nitrogen, Serum creatinine, Malondialdehyde level, marked drop in creatinine clearance Animals which received chrysin reversed all the effects induced by cisplatin in dose dependent manner. Histological studies also substantiated above results. Present study reveals that chrysin attenuated the nephrotoxicity of cisplatin in rats.