Depth-resolved fluorescence of biological tissue

摘要

The depth-resolved autofluorescence of rabbit oral tissue, normal and dysplastic human ectocervical tissue within 120 um depth were investigated utilizing a confocal fluorescence spectroscopy with the excitations at 355 nm and 457 nm. From the topmost keratinizing layer of oral and ectocervical tissue, strong keratin fluorescence with the spectral characteristics similar to collagen was observed. The fluorescence signal from epithelial tissue between the keratinizing layer and stroma can be well resolved. Furthermore, NADH and FAD fluorescence measured from the underlying non-keratinizing epithelial layer were strongly correlated to the tissue pathology. This study demonstrates that the depth-resolved fluorescence spectroscopy can reveal fine structural information on epithelial tissue and potentially provide more accurate diagnostic information for determining tissue pathology.