β-carotene Gingival Mucoadhesive Patch to Prevent Panoramic Radiography Exposure's Effect on GCF

摘要

In the previous study, β-carotene mucoadhesive gingival patch was supposed as a radiation protection agent, but its optimal level still limited to prevent the increasing number of micronucleus on a human. Further study is needed to find the other parameters in gingival mucosa in order to confirm its efficacy. Gingival crevicular fluid (GCF) is an exudate which secreted from gingival mucosa. This study aims to observe the radioprotection effect of β-carotene mucoadhesive gingival patch on GCF after panoramic radiography exposure. Twenty subjects who required a panoramic exposure were divided into two groups, sample, and control. β-carotene gingival mucoadhesive patch was applied on the sample's gingival mucosa of tooth #11 and #21 before panoramic exposure but not applied to the control group. After 5 min exposure, GCF was gathered for each subject from labial side of tooth #11 and #21. Volume was measured after staining by 2 % ninhydrin. The 8-oxo-dG level measurement was done by Enzyme-Linked Immunosorbent Assay (ELISA). Student t-test was used to analyze the statistical difference between groups. Subjects with gingival patch had significantly lower GCF volume and 8-oxo-dG level compared to control subjects (p < 0.05). It is associated with β-carotene antioxidants properties that prevent oxidative reaction also inflammation. The conclusion is β-carotene gingival mucoadhesive patch could be suggested to prevent panoramic radiography exposure effect on GCF.